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    Elabscience Biotechnology mouse il 6 elisa kit
    iMDK alleviates bone loss via dual regulation of bone formation and inflammatory cytokines. (A) Representative micro-CT images of distal femora (top) with three-dimensional reconstruction of the region of interest (bottom), with the blue indicating higher-density bone and the red indicating lower-density bone. (B – D) Statistical quantification of trabecular bone microstructural parameters (BMD, BV/TV, and Tb.Th). Inter-group comparisons were analyzed by one-way ANOVA. (E) Representative images of trabecular bone area in distal femur sections stained with hematoxylin and eosin. Scale bar, 200 μm or 50 μm. (F) Quantitative analysis of the trabecular bone area of the distal femur stained with hematoxylin and eosin. Inter-group comparisons were analyzed by one-way ANOVA. (G) Immunohistochemical staining of OCN in distal femurs. Scale bar, 200 μm or 50 μm. (H) Quantitative analysis of OCN-positive area. Inter-group comparisons were analyzed by one-way ANOVA. (I) Western blotting analysis of inflammatory cytokine expression <t>(IL-6,</t> TNF-α, and IL-1β) in mouse bone tissues. (J) Inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse serum was detected by ELISA. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; “ns” indicates non-significant differences.
    Mouse Il 6 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+interleukin+6+il+6+elisa+kit/Mouse+IL-6+(Interleukin+6)+ELISA+Kit/pmc12886531-44-12-29
    Average 97 stars, based on 714 article reviews
    mouse il 6 elisa kit - by Bioz Stars, 2026-09
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    1) Product Images from "Targeting MDK alleviates bone loss via dual regulation of osteogenic differentiation and inflammatory cytokine expression"

    Article Title: Targeting MDK alleviates bone loss via dual regulation of osteogenic differentiation and inflammatory cytokine expression

    Journal: Genes & Diseases

    doi: 10.1016/j.gendis.2025.101931

    iMDK alleviates bone loss via dual regulation of bone formation and inflammatory cytokines. (A) Representative micro-CT images of distal femora (top) with three-dimensional reconstruction of the region of interest (bottom), with the blue indicating higher-density bone and the red indicating lower-density bone. (B – D) Statistical quantification of trabecular bone microstructural parameters (BMD, BV/TV, and Tb.Th). Inter-group comparisons were analyzed by one-way ANOVA. (E) Representative images of trabecular bone area in distal femur sections stained with hematoxylin and eosin. Scale bar, 200 μm or 50 μm. (F) Quantitative analysis of the trabecular bone area of the distal femur stained with hematoxylin and eosin. Inter-group comparisons were analyzed by one-way ANOVA. (G) Immunohistochemical staining of OCN in distal femurs. Scale bar, 200 μm or 50 μm. (H) Quantitative analysis of OCN-positive area. Inter-group comparisons were analyzed by one-way ANOVA. (I) Western blotting analysis of inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse bone tissues. (J) Inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse serum was detected by ELISA. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; “ns” indicates non-significant differences.
    Figure Legend Snippet: iMDK alleviates bone loss via dual regulation of bone formation and inflammatory cytokines. (A) Representative micro-CT images of distal femora (top) with three-dimensional reconstruction of the region of interest (bottom), with the blue indicating higher-density bone and the red indicating lower-density bone. (B – D) Statistical quantification of trabecular bone microstructural parameters (BMD, BV/TV, and Tb.Th). Inter-group comparisons were analyzed by one-way ANOVA. (E) Representative images of trabecular bone area in distal femur sections stained with hematoxylin and eosin. Scale bar, 200 μm or 50 μm. (F) Quantitative analysis of the trabecular bone area of the distal femur stained with hematoxylin and eosin. Inter-group comparisons were analyzed by one-way ANOVA. (G) Immunohistochemical staining of OCN in distal femurs. Scale bar, 200 μm or 50 μm. (H) Quantitative analysis of OCN-positive area. Inter-group comparisons were analyzed by one-way ANOVA. (I) Western blotting analysis of inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse bone tissues. (J) Inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse serum was detected by ELISA. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; “ns” indicates non-significant differences.

    Techniques Used: Micro-CT, Staining, Immunohistochemical staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

    Recombinant MDK protein triggers the activation of inflammatory cytokines through the NF-κB signaling pathway. (A, B) IL-6, TNFα, and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were treated with recombinant MDK protein (600 ng/mL). Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by a two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (C, D) Western blotting analysis of NF-κB signaling pathway molecules in MC3T3-E1 cells treated with recombinant MDK protein for 7 days during osteoblastic differentiation. Inter-group comparisons were analyzed by two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (E, F) IL-6 and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were pretreated with 10 μM BAY 11–7082. Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.
    Figure Legend Snippet: Recombinant MDK protein triggers the activation of inflammatory cytokines through the NF-κB signaling pathway. (A, B) IL-6, TNFα, and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were treated with recombinant MDK protein (600 ng/mL). Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by a two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (C, D) Western blotting analysis of NF-κB signaling pathway molecules in MC3T3-E1 cells treated with recombinant MDK protein for 7 days during osteoblastic differentiation. Inter-group comparisons were analyzed by two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (E, F) IL-6 and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were pretreated with 10 μM BAY 11–7082. Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.

    Techniques Used: Recombinant, Activation Assay, Expressing, Western Blot, Two Tailed Test

    Schematic representation of MDK alleviating bone loss. MDK is significantly elevated in the serum of postmenopausal osteoporotic women and ovariectomized mice. Due to estrogen deficiency, iMDK alleviates bone loss by promoting bone formation and inhibiting inflammatory factors. Recombinant MDK protein inhibits osteogenic differentiation through the PI3K/AKT signaling pathway and up-regulates inflammatory factors IL-6, TNF-α, and IL-1β via the NF-κB signaling pathway.
    Figure Legend Snippet: Schematic representation of MDK alleviating bone loss. MDK is significantly elevated in the serum of postmenopausal osteoporotic women and ovariectomized mice. Due to estrogen deficiency, iMDK alleviates bone loss by promoting bone formation and inhibiting inflammatory factors. Recombinant MDK protein inhibits osteogenic differentiation through the PI3K/AKT signaling pathway and up-regulates inflammatory factors IL-6, TNF-α, and IL-1β via the NF-κB signaling pathway.

    Techniques Used: Recombinant

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    iMDK alleviates bone loss via dual regulation of bone formation and inflammatory cytokines. (A) Representative micro-CT images of distal femora (top) with three-dimensional reconstruction of the region of interest (bottom), with the blue indicating higher-density bone and the red indicating lower-density bone. (B – D) Statistical quantification of trabecular bone microstructural parameters (BMD, BV/TV, and Tb.Th). Inter-group comparisons were analyzed by one-way ANOVA. (E) Representative images of trabecular bone area in distal femur sections stained with hematoxylin and eosin. Scale bar, 200 μm or 50 μm. (F) Quantitative analysis of the trabecular bone area of the distal femur stained with hematoxylin and eosin. Inter-group comparisons were analyzed by one-way ANOVA. (G) Immunohistochemical staining of OCN in distal femurs. Scale bar, 200 μm or 50 μm. (H) Quantitative analysis of OCN-positive area. Inter-group comparisons were analyzed by one-way ANOVA. (I) Western blotting analysis of inflammatory cytokine expression <t>(IL-6,</t> TNF-α, and IL-1β) in mouse bone tissues. (J) Inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse serum was detected by ELISA. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; “ns” indicates non-significant differences.
    Mouse Il 6 Elisa Kit Boster Bio Cat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Elabscience Biotechnology il 6 elisa kit
    BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α <t>(C),</t> <t>IL-6</t> (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.
    Il 6 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+interleukin+6+il+6+elisa+kit/Mouse+IL-6+(Interleukin+6)+ELISA+Kit/pmc13097173-124-20-24
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    Image Search Results


    iMDK alleviates bone loss via dual regulation of bone formation and inflammatory cytokines. (A) Representative micro-CT images of distal femora (top) with three-dimensional reconstruction of the region of interest (bottom), with the blue indicating higher-density bone and the red indicating lower-density bone. (B – D) Statistical quantification of trabecular bone microstructural parameters (BMD, BV/TV, and Tb.Th). Inter-group comparisons were analyzed by one-way ANOVA. (E) Representative images of trabecular bone area in distal femur sections stained with hematoxylin and eosin. Scale bar, 200 μm or 50 μm. (F) Quantitative analysis of the trabecular bone area of the distal femur stained with hematoxylin and eosin. Inter-group comparisons were analyzed by one-way ANOVA. (G) Immunohistochemical staining of OCN in distal femurs. Scale bar, 200 μm or 50 μm. (H) Quantitative analysis of OCN-positive area. Inter-group comparisons were analyzed by one-way ANOVA. (I) Western blotting analysis of inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse bone tissues. (J) Inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse serum was detected by ELISA. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; “ns” indicates non-significant differences.

    Journal: Genes & Diseases

    Article Title: Targeting MDK alleviates bone loss via dual regulation of osteogenic differentiation and inflammatory cytokine expression

    doi: 10.1016/j.gendis.2025.101931

    Figure Lengend Snippet: iMDK alleviates bone loss via dual regulation of bone formation and inflammatory cytokines. (A) Representative micro-CT images of distal femora (top) with three-dimensional reconstruction of the region of interest (bottom), with the blue indicating higher-density bone and the red indicating lower-density bone. (B – D) Statistical quantification of trabecular bone microstructural parameters (BMD, BV/TV, and Tb.Th). Inter-group comparisons were analyzed by one-way ANOVA. (E) Representative images of trabecular bone area in distal femur sections stained with hematoxylin and eosin. Scale bar, 200 μm or 50 μm. (F) Quantitative analysis of the trabecular bone area of the distal femur stained with hematoxylin and eosin. Inter-group comparisons were analyzed by one-way ANOVA. (G) Immunohistochemical staining of OCN in distal femurs. Scale bar, 200 μm or 50 μm. (H) Quantitative analysis of OCN-positive area. Inter-group comparisons were analyzed by one-way ANOVA. (I) Western blotting analysis of inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse bone tissues. (J) Inflammatory cytokine expression (IL-6, TNF-α, and IL-1β) in mouse serum was detected by ELISA. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; “ns” indicates non-significant differences.

    Article Snippet: We purchased the Mouse P1NP (Procollagen 1 N-Terminal Propeptide) ELISA Kit (Cat#e-el-m0233), Mouse IL-6 ELISA Kit (Cat#e-el-m0044), Mouse TNF-α ELISA Kit (Cat#e-el-m3063), and Mouse IL-1β ELISA Kit (Cat#e-el-m0037) from Elabscience (Wuhan, China).

    Techniques: Micro-CT, Staining, Immunohistochemical staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

    Recombinant MDK protein triggers the activation of inflammatory cytokines through the NF-κB signaling pathway. (A, B) IL-6, TNFα, and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were treated with recombinant MDK protein (600 ng/mL). Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by a two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (C, D) Western blotting analysis of NF-κB signaling pathway molecules in MC3T3-E1 cells treated with recombinant MDK protein for 7 days during osteoblastic differentiation. Inter-group comparisons were analyzed by two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (E, F) IL-6 and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were pretreated with 10 μM BAY 11–7082. Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.

    Journal: Genes & Diseases

    Article Title: Targeting MDK alleviates bone loss via dual regulation of osteogenic differentiation and inflammatory cytokine expression

    doi: 10.1016/j.gendis.2025.101931

    Figure Lengend Snippet: Recombinant MDK protein triggers the activation of inflammatory cytokines through the NF-κB signaling pathway. (A, B) IL-6, TNFα, and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were treated with recombinant MDK protein (600 ng/mL). Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by a two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (C, D) Western blotting analysis of NF-κB signaling pathway molecules in MC3T3-E1 cells treated with recombinant MDK protein for 7 days during osteoblastic differentiation. Inter-group comparisons were analyzed by two-tailed unpaired Student's t -test (for normally distributed data with equal variance). (E, F) IL-6 and IL-1β expression levels were detected using Western blotting. MC3T3-E1 cells were pretreated with 10 μM BAY 11–7082. Osteogenic differentiation was induced for 7 days. Inter-group comparisons were analyzed by one-way ANOVA. ∗ p < 0.05 and ∗∗ p < 0.01.

    Article Snippet: We purchased the Mouse P1NP (Procollagen 1 N-Terminal Propeptide) ELISA Kit (Cat#e-el-m0233), Mouse IL-6 ELISA Kit (Cat#e-el-m0044), Mouse TNF-α ELISA Kit (Cat#e-el-m3063), and Mouse IL-1β ELISA Kit (Cat#e-el-m0037) from Elabscience (Wuhan, China).

    Techniques: Recombinant, Activation Assay, Expressing, Western Blot, Two Tailed Test

    Schematic representation of MDK alleviating bone loss. MDK is significantly elevated in the serum of postmenopausal osteoporotic women and ovariectomized mice. Due to estrogen deficiency, iMDK alleviates bone loss by promoting bone formation and inhibiting inflammatory factors. Recombinant MDK protein inhibits osteogenic differentiation through the PI3K/AKT signaling pathway and up-regulates inflammatory factors IL-6, TNF-α, and IL-1β via the NF-κB signaling pathway.

    Journal: Genes & Diseases

    Article Title: Targeting MDK alleviates bone loss via dual regulation of osteogenic differentiation and inflammatory cytokine expression

    doi: 10.1016/j.gendis.2025.101931

    Figure Lengend Snippet: Schematic representation of MDK alleviating bone loss. MDK is significantly elevated in the serum of postmenopausal osteoporotic women and ovariectomized mice. Due to estrogen deficiency, iMDK alleviates bone loss by promoting bone formation and inhibiting inflammatory factors. Recombinant MDK protein inhibits osteogenic differentiation through the PI3K/AKT signaling pathway and up-regulates inflammatory factors IL-6, TNF-α, and IL-1β via the NF-κB signaling pathway.

    Article Snippet: We purchased the Mouse P1NP (Procollagen 1 N-Terminal Propeptide) ELISA Kit (Cat#e-el-m0233), Mouse IL-6 ELISA Kit (Cat#e-el-m0044), Mouse TNF-α ELISA Kit (Cat#e-el-m3063), and Mouse IL-1β ELISA Kit (Cat#e-el-m0037) from Elabscience (Wuhan, China).

    Techniques: Recombinant

    BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    BAI mitigated the inflammation of IMN mice. (A) After being treated with 20 mg/kg and 100 mg/kg BAI, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) After being treated with BAI, the level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) After being treated with BAI, the serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI mitigated the inflammation of IMN mice. (A) After being treated with 20 mg/kg and 100 mg/kg BAI, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) After being treated with BAI, the level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) After being treated with BAI, the serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay

    BAI suppressed the inflammation and oxidative stress of ZAS-induced podocytes by inactivating the AGE/RAGE pathway. (A-B) After being 10% ZAS induced for 1 h and 2 µmol/L BAI treated for 24 h, the expression of AGE (A) and RAGE (B) in MPC-5 cells were quantified. (C) After being treated with 2 µmol/L BAI and 2 µg/mL AGE-BSA for 24 h, the expression of RAGE in ZAS-induced MPC-5 cells were quantified using western blot assay. (D) The viability of ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment was determined using CCK-8 assay. (E-G) The levels of pro-inflammatory cytokines TNF-α (E), IL-6 (F), and MCP-1 (G) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. (H-I) The level of T-SOD (H) and MDA (I) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI suppressed the inflammation and oxidative stress of ZAS-induced podocytes by inactivating the AGE/RAGE pathway. (A-B) After being 10% ZAS induced for 1 h and 2 µmol/L BAI treated for 24 h, the expression of AGE (A) and RAGE (B) in MPC-5 cells were quantified. (C) After being treated with 2 µmol/L BAI and 2 µg/mL AGE-BSA for 24 h, the expression of RAGE in ZAS-induced MPC-5 cells were quantified using western blot assay. (D) The viability of ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment was determined using CCK-8 assay. (E-G) The levels of pro-inflammatory cytokines TNF-α (E), IL-6 (F), and MCP-1 (G) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. (H-I) The level of T-SOD (H) and MDA (I) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: Expressing, Western Blot, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

    BAI mitigated the inflammation and oxidative stress of IMN mice via AGE/RAGE pathway. (A) After being treated with 20 mg/kg BAI and 20 mg/kg AGE-BSA, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) The level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) The serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. (F) The LDH level in the kidney tissue of IMN mice was detected using a commercial reagent kit. (G-H) The levels of T-SOD (G) and MDA (H) in kidney tissue of IMN mice was detected by ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). ** p < 0.01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI mitigated the inflammation and oxidative stress of IMN mice via AGE/RAGE pathway. (A) After being treated with 20 mg/kg BAI and 20 mg/kg AGE-BSA, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) The level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) The serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. (F) The LDH level in the kidney tissue of IMN mice was detected using a commercial reagent kit. (G-H) The levels of T-SOD (G) and MDA (H) in kidney tissue of IMN mice was detected by ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). ** p < 0.01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay